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A limitation of this study is that the biopsies were not all performed at one institution, which could cause error due to diagnostic bias. The study population was limited to women with abnormal cervicography, which makes it difficult to assess the true disease status and could cause verification bias in evaluating the diagnostic device [23, 24].. The expression of phospho-apoptosis signal-regulating kinase-1 (pASK-1), phospho-p38MAPK, and phosphor-NF-κB p65 was evaluated by western blot after 12 hours of reperfusion. The ischemia group (Group C) showed that I/R injury increased phosphorylation of ASK-1, p38, and NF-κB. In the ischemia-PRP group (Group D), PRP significantly reduced pASK-1 expression, indicating that PRP could protect flaps from I/R injury by suppressing ASK-1 (Figure 8A and B). Although phospho-p38MAPK was slightly decreased in Group D, this difference was not statistically significant (p > 0.05). The PRP group (Group B) exhibited a slight decrease in phospho-p38 compared with the control group (Group A); however, this difference was not statistically significant. Non-I/R injury groups showed lower levels of phospho-p38 than I/R injury groups. (Figure 8C and 8D). I/R injury increased the activity of pNF-κB in Group C. However, PRP treatment reduced the expression of pNF-κB in Group D, displaying apparent degradation of IκB (Figure 8E and 8F).

The expression of phospho-apoptosis signal-regulating kinase-1 (pASK-1), phospho-p38MAPK, and phosphor-NF-κB p65 was evaluated by western blot after 12 hours of reperfusion. The ischemia group (Group C) showed that I/R injury increased phosphorylation of ASK-1, p38, and NF-κB. In the ischemia-PRP group (Group D), PRP significantly reduced pASK-1 expression, indicating that PRP could protect flaps from I/R injury by suppressing ASK-1 (Figure 8A and B). Although phospho-p38MAPK was slightly decreased in Group D, this difference was not statistically significant (p > 0.05). The PRP group (Group B) exhibited a slight decrease in phospho-p38 compared with the control group (Group A); however, this difference was not statistically significant. Non-I/R injury groups showed lower levels of phospho-p38 than I/R injury groups. (Figure 8C and 8D). I/R injury increased the activity of pNF-κB in Group C. However, PRP treatment reduced the expression of pNF-κB in Group D, displaying apparent degradation of IκB (Figure 8E and 8F)..

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The diagram can be properly utilized to choose the best scaffold geometry that is better suited for the specific patient requirements. Let us suppose, for example, that a value of Lpatient = 0,35 MPa was found (Figure 4). It appears that the best scaffold geometry that can be selected by the surgeon is the one with rectangular pores and a ratio A/B = 0.5. The second best solution is a scaffold with elliptic pores and with A/B = 0.75..

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